Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts.
نویسندگان
چکیده
Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 73 18 شماره
صفحات -
تاریخ انتشار 2007